Coding
GroES

Part:BBa_K205001:Design

Designed by: Richard Schlegel   Group: iGEM09_UQ-Australia   (2009-09-08)

GroES is a chaperone protein that prevents polypeptide misfolding.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 89
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 89
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 89
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene was provided by Christos Kyratsous.


Source

Christos Kyratsous Columbia Univ, Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA Ref: Kyratsous, CA, Silverstein, SJ, DeLong, CR, Panagiotidis, CA. (2009) "Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli" GENE VOL: 440 Issue: 1-2 Pages: 9-15 Date: Jul 1 2009

References

GroES: http://www.uniprot.org/uniprot/P0A6F9