Coding
GroES
Part:BBa_K205001:Design
Designed by: Richard Schlegel Group: iGEM09_UQ-Australia (2009-09-08)
GroES is a chaperone protein that prevents polypeptide misfolding.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 89
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 89
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 89
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 89
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Gene was provided by Christos Kyratsous.
Source
Christos Kyratsous Columbia Univ, Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA Ref: Kyratsous, CA, Silverstein, SJ, DeLong, CR, Panagiotidis, CA. (2009) "Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli" GENE VOL: 440 Issue: 1-2 Pages: 9-15 Date: Jul 1 2009
References
GroES: http://www.uniprot.org/uniprot/P0A6F9